Provided by Peak Surgicals is a Sterilization Box. It must therefore be possible to sanitize surfaces coming into contact with humans and other bacteria that are pathogenic as well as the COVID-19 virus. The main aim of this study was to create an inexpensive prototype of a sterilization box for small things which use heat and ultraviolet (UV) radiation. Two studies were conducted to evaluate performance.

Firstly, step one involved sterilizing IgG (a model protein identical to the spike glycoprotein of the SARS-COV-2) in an incubator using UV and heat. In 15 min at 70 °C, the protein was efficiently unfolded and then aggregated by UV exposure during incubation. Under optimal conditions, hydrodynamic size of the protein increased from 5 nm of native protein to 171 nm.

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 In addition, similar observations were made with regards to OD280 readings where they were observed to rise from 0.17 to 0.78 indicating that more aromatic moieties within it became exposed as a result of unfolding. Furthermore, intrinsic fluorescence measurement as well as FTIR analysis showed that there was a 70% increase in -sheets while -helixes decreased by approximately22%, molecular changes consistent with unfolding and aggregation.

This box could destroy SARS-COV-2 through changing its natural form according to the constructed item’s implication on the structures of proteins produced by living organisms, including humans or animals. Also, both E.coli clinical isolates and those obtained from everyday objects demonstrated complete antibacterial activity after just quarter-hour inside chamber under 70 °C standard temperatures for incubations It is the first comprehensive performance study on the effectiveness of combining heat and UV irradiation to kill bacteria and viruses.

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